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Organisms this product works with:
Other products used in the isolation of Escherichia coli:
ENTEROBACTERIA ENRICHMENT BROTH-MOSSEL (EP/USP/JP/BP)
Code: CM1115
an enrichment medium for bile-tolerant Gram-negative bacteria in the microbiological examination of pharmaceutical products
Typical Formula | gm/litre |
Pancreatic digest of gelatine | 10.0 |
Glucose anhydrous | 4.5† |
Bile Salts (Sodium desoxycholate, Sodium lauryl sulphate, Sodium citrate) | 3.055†† |
Potassium dihydrogen phosphate | 2.0 |
Disodium hydrogen phosphate anhydrous | 6.4††† |
Brilliant green | 0.015 |
pH 7.2 ± 0.2 @ 25°C |
Directions
Dissolve 25.97g in 1 litre of purified water. Mix well and distribute 100ml into 250ml flasks. Heat the medium at 100°C using free flowing steam for 30 minutes only. Cool rapidly in cold running water. DO NOT AUTOCLAVE.
Description
Enterobacteria Enrichment Broth-Mossel (EP/USP/JP/BP) is recommended as an enrichment medium for bile-tolerant Gram-negative bacteria in the microbiological examination of pharmaceutical products. It can be used as test for absence or quantitative test1.
Oxoid Enterobacteria Enrichment Broth-Mossel (EP/USP/JP/BP) was formulated to overcome the unsatisfactory effects of inhibition on small numbers of bile-tolerant gram negative cells due to bile salt variations. The inclusion of purified ox bile eliminated these problems and a preliminary assay can be used to check growth by inoculating approximately one viable cell per medium unit2,3.
Enterobacteria Enrichment Broth-Mossel (EP/USP/JP/BP) should be used as an enrichment broth in conjunction with Violet Red Bile Glucose Agar (R455302).
Additional information about this and other products in our harmonised range can be obtained by downloading our Harmonised Culture Media Portfolio.
Technique
| Probable number of bacteria per gram or millilitre of product | ||
0.1g or 0.1ml | 0.01g or 0.01ml | 0.001g or 0.001ml | |
+ | + | + | >103 |
- | + | + | <103 and >102 |
- | - | + | <102 and >101 |
- | - | - | <10 |
Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
Store the prepared medium at 2-25°C.
Appearance
Dehydrated medium: Pale green, free-flowing powder
Prepared medium: Green solution
Quality control
Inoculate the test strains individually. Use a number of micro-organisms equivalent to not more than 100 CFU in the inoculated test preparation.
Positive controls: | Expected results† |
Escherichia coli ATCC® 8739 | Good growth |
Pseudomonas aeruginosa ATCC® 9027 | Good growth |
Negative control: | |
Staphylococcus aureus ATCC® 6538 | Inhibited |
References
1. European Pharmacopoeia 6.1. 2.6.13 Microbiological Examination of Non-Sterile Products: Test for Specified Micro-organisms. B. Harmonised Method.
2. Mossel D. A. A., Harrewijn G. A. and Nesselrooy-van Zadelhoff C. F. M. (1974) Health Lab. Sci. 11. 260-267.
3. Richard N. (1982) in Quality Assurance and quality control of microbiological culture media. Ed. J.E.L. Corry. G.I.T. - Verlag Darmstadt. pp 51-57.