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Diagnostic Discs

FURAZOLIDONE DISCS

Code: DD0028

for the differentiation of micrococci and staphylococci

Contents
5 cartridges, each containing 50 discs (250 discs per pack)

Description
Furazolidone susceptibility testing is a reliable method for the differentiation of micrococci (which are resistant giving little or no zone of inhibition around the disc) and staphylococci (which are sensitive). In a study by John Baker1 100% of the micrococci tested were resistant, and 99.5% of the staphylococci were susceptible.

Technique
Prepare a suspension of the test organism equivalent to a 0.5 McFarland turbidity standard. Inoculate the suspension evenly onto a Mueller-Hinton agar plate (CM0337), apply the furazolidone disc and incubate at 35-37°C for 18-24 hours. After incubation micrococci will give a zone of <9mm and staphylococci will give zones ≥15mm.

Quality Control
Organism

Expected zone size

Staphylococcus aureus  ATCC® 25923*

20 – 28mm

Micrococcus spp. ATCC® 10240

<9mm

* This organism is available as a Culti-Loop®

Reference
1. J. Baker 1984 J. Clin. Micro Vol 19, No 6: P875 - 879

 
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