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Material Safety Data Sheet


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Molecular Microbiology

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Code: QB0673C

BAX System PCR Multiplex Assay for rapid detection of Escherichi coli 0157/H7

Escherichi coli 0157/H7

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Certain strains of Esherishia coli can produce an  enterotoxin.  This toxin can cause severe, acute and debilitating diseases such as Haemolytic Uraemic Syndrome (HUS). E. coli O157:H7 is the most commonly implicated strain; cattle and beef products are frequently implicated in the transmission of this organism.

When E. coli O157:H7 is present in a food sample, the genetic-based BAX System uses the power of the polymerase chain reaction (PCR) with this multiplex assay to rapidly create (amplify) millions of copies of DNA fragments that are specific to E. coli O157:H7. This is a multiplex assay.  That means that several different, but specific E. coli O157:H7 primers are used.  If any one of these primers recognizes target DNA in the sample, then it will be amplified, thereby increasing your assurance that new, unusual or modified varieties of this complex organism are detected.

BAX System assays simplify the method by combining all the PCR reagents you need into a single tablet, conveniently packaged inside the PCR tubes contained in each kit. Reducing the number of manual manipulations and pipetting steps, the tabletted reagents improve accuracy and consistency; hands-on time is reduced to a minimum and shelf life is increased.

After standard enrichment, samples are lysed to break open the cell walls and release DNA. This lysate is then used to hydrate the PCR tablets. Processing in the automated thermo-cycler takes less than 4 hours for a full rack of 96 tests. Electronic results appear as positive/negative icons on-screen, and the data can be easily stored, shared or printed.

The BAX System multiplex (MP) assay for E. coli O157:H7 detects both standard and unusual varieties of E. coli O157:H7 in beef. Validation studies have demonstrated that the BAX System MP assay, after 8-24 hours enrichment in BAX E. coli O157:H7 MP media (CM1076), performs significantly better than the USDA-FSIS culture method for detecting low levels of E. coli O157:H7 in raw ground beef. Performance between the methods was comparable for beef trim samples. With sensitivity/specificity rates of >99%, the BAX System for detecting E. coli O157:H7 MP assay has been adopted by the USDA-FSIS and certified by AOAC Research Institute as a Performance Tested method.

Media for Use with BAX Escherichia coli 0157:H7

 Medium Code
 BAX E. coli 0157:H7 MP Broth  CM1076
 Modified EC Broth CM1037
 Novobiocin Supplement - 10mg/vial SR0181

USDA-FSIS MLG 5A.00 - raw ground beef and raw beef trim

Health Canada Supplement 2 to MFLP#30 - applies to dairy, meat, fruit and vegetables (after 18-24 hours in modified tryptic soy broth with novobiocin, mTSB-n) and to dry foods, environmentals and animal feed (after 1 hour in BPW, then 18-24 hours in mTSB-n). Also applies to ground beef and beef trim after 8-24 hours in BAX System MP medium.

Bax System Q7

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