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Material Safety Data Sheet

Further Information

Optional Products

This item can be used optionally with:

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Organisms this product works with:

For this Organism

Other products used in the isolation of Streptococci:

Diagnostic Reagents

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STREPTOCOCCAL GROUPING KIT

CODE: DR0585

A latex agglutination test for the identification of streptococcal groups A, B, C, D, F and G.

Introduction
Lancefield showed that the majority of pathogenic streptococci possess specific carbohydrate antigens, which permit the classification of streptococci into groups. These streptococcal group antigens can be extracted from the cells and their presence demonstrated with latex particles previously coated with group-specific antibodies. These latex particles will agglutinate in the presence of homologous antigen, but will remain in smooth suspension in the absence of such antigen. The Oxoid Streptococcal Grouping Kit is such a latex agglutination test for the identification of the streptococcal group and reagents are provided for groups A, B, C, D, F and G. The use of a enzymatic extraction procedure considerably shortens the time required for antigen extraction and much improves the antigen yield, partially for Group D streptococci.

Components of the Kit
DR0586 Latex Group Reagent A
DR0587 Latex Group Reagent B
DR0588 Latex Group Reagent C
DR0589 Latex Group Reagent D
DR0590 Latex Group Reagent F
DR0591 Latex Group Reagent G
DR0592 Polyvalent Positive Control
DR0593 Extraction Enzyme
DR0500 Disposable reaction cards.

Storage
The Oxoid Streptococcal Grouping Kit should be stored at 2-8°C. Do not freeze. The kit should not be used after the expiry date printed on the outside of the carton.

Precautions
Each latex reagent contains 0.1% sodium azide.
The positive control reagent contains 0.1% sodium azide.
After reconstitution the extraction enzyme solution contains 0.01% thiomersal.

References
1. Lancefield R.C. (1938) Proc. Soc. Exp. Bio. Med. 38, 473.
2. Facklam R.R. (1980) in ’Manual of Clinical Microbiology’, 3rd Edition. American Society for Microbiology, Washington, D.C., pp.88-110.
3. Mcllmurray M.B. (1984) Lancet, 1, 1353.
4. Birch B.R., Keaney M.G.L. and Ganguli L.A. (1984) Lancet, 1, 856-857.
5. Facklam R.R. and Carey R.B. (1985) in ’Manual of Clinical Microbiology’, 4th Edition. Eds.Lennette E.H., Balows A., Hausler W.J., Shadomy H.J., Amer. Soc. for Microbiol., Washington, D.C., pp. 154-175.
6. Kloos W.E., and Jorgensen J.H. (1985) in ’Manual of Clinical Microbiology’, 4th Edition, pp. 143-153.
7. Bortolussi R., Schelech W.F. and Albritton W.L. (1985) in ’Manual of Clinical Microbiology’, 4th Edition, pp. 205-208.

 
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