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• Anaerobic Systems
• • How do I carry out the evacuation/replacement technique using the Oxoid Anaerobic Jar?
  • How do I use an Anaerobic Jar with a Gas Generating Kit?
  • How should I clean my anaerobic jar?
• Antibiotic Sensitivity Testing
• Culture Media, Supplements and Raw Materials
• Diagnostic Reagents
• Dipslides
• General
• Toxin Detection

1. Place the Petri dishes in the carrier.
Disposable plastic Petri dishes should be of the vented variety to aid gas transfer between the interior and exterior of the dishes.

When performing an anaerobic incubation, use an Oxoid Anaerobic Gas Generating Kit (BR0038) and Oxoid Anaerobic Indicator (BR0055). This Gas Generating Kit will provide the correct atmosphere in which to effect culture anaerobes. For the culture of microaerophilic organisms, use an Oxoid Campylobacter Gas Generating Kit. Since the proportion of oxygen to carbon dioxide is critical when growing micreoaerophiles, ensure that the correct kit is used. Two products are available, and are designated by the codes BR0056 and BR0060.

BR0056 is designed for use in jars with a capacity of 3.0 to 3.5 litres and BR0060 for use in jars with a capacity of 2.5 litres.
CAUTION: ENSURE THE CORRECT KIT IS USED.

2. Cut open an Oxoid Anaerobic Indicator (BR0055) and expose 10mm of the fabric strip. Insert the sachet into a smaller, upper clip on the dish carrier.

On opening, the indicator strip should be slightly moist. It may also appear slightly pink. Under normal circumstances, this will not lead to inferior performance. However, should the strip appear deep pink in colour on opening, please take advice from Oxoid Ltd.

3. Lower the dish carrier into the polycarbonate base.

4. Cut off the corner of an Oxoid Gas Generating Kit (BR0038) as indicated by the broken line. Add 10ml of water to the sachet and immediately place in the lower clip of the dish carrier.

Avoid folding or crushing the sachet.

5. Place the lid on the base, making sure that the O-ring is correctly in place, evenly pressed against the flange as a secure fit. Apply the beam clamp. This is easily done by the first holding the beam over the flange on the lid and parallel with the two Schrader valves. Move it horizontally with the top of the beam slightly inclined towards the gauge and safety valve, so that the screw thread passes between the two Schrader valves to the location point on the lid centre. Screw down the knurled wheel until tight.

6. Observe the pressure gauge. The evolution of gas will produce a positive pressure up to approximately 0.3 bar. The pressure will fall over a period of 30-40 minutes to 0.1 bar approximately. The pressure will not fall in the presence of an inactive catalyst.

7. After 2-3 hours the Anaerobic Indicator will change from pink to white. This will give a visual indication of anaerobiosis.

8. After incubation the Anaerobic Indicator may be discarded with the normal laboratory litter. The exhausted Gas Generating Kit should be removed without spilling the contents. The solution left in the sachet is mildly acid and may be poured away into the sink and flushed with running water. The empty sachet can be discarded with normal laboratory litter.

9. To increase the rate of activity, the catalyst should be dried between uses by heating to 160°C for 90 minutes. This regeneration procedure between uses will allow the catalyst to be used for up to 30 cycles.

10. Store in the jar with the lid invested so that the catalyst will remain dry when not in use. The interior of the lid and jar should be kept free of dust.