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Dehydrated Culture Media

MALT EXTRACT BROTH

Code: CM0057

A liquid medium recommended for the cultivation of moulds and yeasts, especially during tests for sterility.

Typical Formula*

gm/litre

Malt extract

17.0

Mycological peptone

3.0

pH 5.4 ± 0.2

* Adjusted as required to meet performance standards

Directions
Add 20g to 1 litre of distilled water. Mix well, distribute into final containers and sterilise by autoclaving at 115°C for 10 minutes. This liquid medium is recommended for the cultivation of moulds and yeasts, during tests for sterility, etc.

Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
Store the prepared medium at 2-8°C.

Appearance
Dehydrated medium: Straw coloured, free-flowing powder.
Prepared medium: Amber coloured solution.

Quality control

Positive controls:

Expected result

Aspergillus niger ATCC® 9642 *

Surface growth; white mycelium, black spores

Candida albicans ATCC® 10231 *

Turbid growth

Negative control:

 

Uninoculated medium

No change

* This organism is available as a Culti-Loop®

Reference
1. Galloway L. D. and Burgess R. (1952) `Applied Mycology and Bacteriology’ 3rd ed., Leonard Hill, London, pp. 54 and 57.

 
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