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• Toxin Detection
• • Are there any special protocols for testing fatty foods when using Oxoid Toxin Detection kits?
  • Do I need a centrifuge to carry out the Oxoid C. difficile Toxin A test?

OXOID TECHNICAL SUPPORT DEPARTMENT GUIDELINES

TOXIN DETECTION FROM FATTY FOODS

Food samples which are high in fat e.g. cheese may require a slightly amended protocol when testing with Oxoid Toxin Detection kits.

1. Add 10 grams of the food sample to 10 mL of saline and homogenise.
2. Adjust the suspension to pH 4.0 with 4N hydrochloric acid.
3. Centrifuge and filter as per kit instructions.
4. Adjust the supernatant/filtrate to pH 6-8 and use in RPLA test.