Part of Thermo Fisher Scientific
OXOID C. DIFFICLE TOXIN A STOOL FILTRATION SYSTEM
Code: TD0975
INTENDED USE
The Oxoid stool filtration system is an alternative preparation technique for
the isolation of C. difficle toxin A from stool samples prior to testing
with the Oxoid C. difficle Toxin A Test (TD0970). The system is intended
exclusively for for use with the Oxoid C. difficle Toxin A Test and does
not require the use of a centrifuge.
SAMPLE PREPARATION
1. Note: All components and reagents should
be allowed to reach room temperature
(18-30° C) for 30 minutes before use.
2. a) Liquid Stools - Pippette 1ml of sample diluent to the sample cup of the stool filtration system. Using a pipette, add 100m of stool to the sample diluent in the the sample cup. b) Solid or Semi-Solid Stools - Pippette 1ml of sample diluent to the sample cup. Using a loop, add a 5mm diameter bead-like portion of stool into the sample cup.
3. Mix the sample thoroughly using a pipette or loop, as appropriate, to form an even suspension.
4. Place the filter body (with cap in place) into the sample cup, and push downward on the cap so that the filter body stops approximately 2mm above the level of the liquid.
5. Allow the particulate matter to settle by standing the stool filter system on a level surface for 1 minute.
6. Apply further pressure to the cap of the filter body, and continue to push downwards so that the sample passes through the filter into the filter body.
TEST PROCEDURE
Remove the toxin test unit from the foil puch and place on a level
surface. Remove the cap from the body of the filter unit. Invert
the stool filtration
system and apply 6 drops of the stool filtrate to the sample
window of the C. difficle Toxin A Test unit, by squeezing the sides of
the
nozzle.
INTERPRETATION
OF RESULTS
Following application of sample from the stool filtration system to the test
device results should be interpreted as directed in the C.
difficle Toxin A Test product insert.