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• Anaerobic Systems
• Antibiotic Sensitivity Testing
• Culture Media, Supplements and Raw Materials
• • Are there any guidelines for preparation and reconstitution of culture media?
  • Are there any guidelines for sterilising culture media?
  • Can I prepare culture media in a microwave?
  • Can I store selective supplements after reconstitution?
  • How can I re-melt solid media?
  • How long can I store culture media after preparation?
  • How should I dispose of used culture media?
  • How should I test the pH of culture media?
  • My culture medium does not look right - what has gone wrong?
  • My culture medium is not performing correctly - what has gone wrong?
  • Should I boil media before autoclaving?
  • What are the main components of culture media?
  • What exactly is agar?
  • What grade of water should I use to prepare culture media?
  • What is the shelf life of dehydrated culture media after the pot has been opened?
  • What quality control testing should I carry out on culture media?
  • Which blood product should I choose to make blood agar?
• Diagnostic Reagents
• Dipslides
• General
• Toxin Detection

STORAGE OF RECONSTITUTED SUPPLEMENTS

Storage of reconstituted supplements is not officially recommended, however, we do recognise that there are circumstances where customers wish to do this.

In these situations our advice is to freeze aliquots of the freshly reconstituted supplement at -20°C. When stored in this way the aliquots should remain stable for several months.

Each aliquot should then be re-thawed only once and used immediately.

No data is available to substantiate the validity of this procedure, as the variation in freezing speed, aliquot size etc. between laboratories cannot be accounted for. In addition, certain supplements are not suitable to undergo this procedure.

Customers should check the performance of the frozen aliquots against freshly reconstituted supplement, and produce their own guidelines for the shelf life of each supplement stored in this way.